Fig_3.tif (255.62 kB)
The effect of a high-glucose state on DNMT and TET in INS-1 cells.
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posted on 2015-02-06, 03:56 authored by Kota Ishikawa, Shin Tsunekawa, Makoto Ikeniwa, Takako Izumoto, Atsushi Iida, Hidetada Ogata, Eita Uenishi, Yusuke Seino, Nobuaki Ozaki, Yoshihisa Sugimura, Yoji Hamada, Akio Kuroda, Keiko Shinjo, Yutaka Kondo, Yutaka Oiso(A-D) INS-1 cells were cultured under normal-culture-glucose (11.2 mmol/l; white bar) or experimental-high-glucose (22.4 mmol/l; black bar) conditions for 14 days. The Dnmt (A) and Tet (C) mRNA levels were examined by real-time PCR. DNA methyltransferase (DNMT) (B) and ten-eleven-translocation methylcytosine dioxygenase (TET) (D) activities were examined by ELISA. All results are mean ± SEM (n ≥ 4). Asterisks indicate statistically significant difference (*p < 0.05, **p < 0.01).
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DNA methylation inhibitorDNA methyltransferase activityIns 1 promoterdna methylationinsulin mRNA suppressionrat insulin 1 geneinsEndoplasmic Reticulum StressPalmitate Induces DNA MethylationcAMP response elementrat pancreatic beta cell linepancreatic beta cellsRepresses Transcriptional ActivityMetformin upregulated insulin gene expressioninsulin promoter DNA methylationInsulin Gene Promoterectopic triacylglycerol accumulation
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