The effect of E2 on telomerase activity and hTERT expression in the ER-positive Ishikawa cell line.

Cells were treated with different concentrations of E2 (0.1–10 µM) for 48 hr or treated with 1 uM E2 in a time-course fashion (A). Telomerase activity was determined by the TRAP assay. hTERT RNA expression was assessed by real-time RT-PCR (B). The data are presented as means ± SD of duplicated samples from at least two independent experiments. (ITAS = internal telomerase assay standard, C = control).