Figure_1.tif (1.28 MB)
The effect of E2 on telomerase activity and hTERT expression in the ER-positive Ishikawa cell line.
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posted on 2013-02-19, 15:41 authored by Chunxiao Zhou, Tara A. Steplowski, Hallum K. Dickens, Kimberly M. Malloy, Paola A. Gehrig, John F. Boggess, Victoria L. Bae-JumpCells were treated with different concentrations of E2 (0.1–10 µM) for 48 hr or treated with 1 uM E2 in a time-course fashion (A). Telomerase activity was determined by the TRAP assay. hTERT RNA expression was assessed by real-time RT-PCR (B). The data are presented as means ± SD of duplicated samples from at least two independent experiments. (ITAS = internal telomerase assay standard, C = control).