The effect of AKAP12 on the growth of human primary xenografts.

(A) AKAP12 suppresses the growth of LoVo cells implanted in BALB/c nude mice. Mice injected with LoVo-AKAP12 cells displayed significantly reduced tumor volume compared with the animals injected with LoVo-CON or LoVo-LIPO cells four weeks after injection. (B) Tumor size was measured every week. Each point represents the mean volume ± SD on the X-axis. *p<0.01, LoVo-AKAP12 versus LoVo-CON versus LoVo-LIPO. p<0.01, LoVo-AKAP12 versus LoVo-CON. (C) Effect of AKAP12 expression on the spontaneous metastasis of LoVo cells in nude mice. The quantitative real-time PCR amplification of the human Alu sequence and mouse GAPDH was performed using DNA extracts from the lung. (D) Western blot analysis of AKAP12 and cleaved-caspase-3 level in Lovo-CON and Lovo-AKAP12 group. AKAP12 and cleaved-caspase-3 were found to increase in the Lovo-AKAP12 group.