The effect of AICAR on pAMPK and mitotracker red stain in control and patient fibroblasts.

Control and patient fibroblasts were grown on coverslips in GLU or GAL medium and in GAL supplemented with 0.5 mM AICAR (GAL+AICAR) for 72 hrs. Cells were than incubated with mitotracker red (MTR), fixed, stained with anti pAMPK antibodies and visualized by fluorescent (pAMPK) secondary antibodies. The coverslips were examined by confocal fluorescent microscopy (10×40). A: depicts a representative migrograph of pAMPK stain in green and MTR stain in red. The graphs represent green intensity per cell (B) and red intensity per cell (C) +/− standard deviation (*p<0.05). All micrographs were taken under the same conditions.