The correlation between AHR and MYCN mRNA expression level and differentiation histology in tumor samples of patients with NB.

(A) Total mRNA of patient tumor samples were isolated by TRIzol reagent. The mRNA expression level of AHR and MYCN were analyzed using SYBR-green real-time PCR using gene specific primers. (B) The inverse correlation were analyzed by Spearman’s correlation test (Spearman’s ρ = −0.6484, p = 0.0121) (C) AHR expression level was analyzed in tumors with high and low MYCN expression status. (D) The total mRNA of MYCN non-amplified NB cell lines, SY5Y and SK-N-SH, and MYCN amplified NB cell lines, SK-N-BE, SK-N-DZ, and IMR32, were collected and subjected to SYBR-green real-time PCR with specific primers for AHR and MYCN. (E) The correlation between AHR expression level and differentiation of NB tumor histology was analyzed in 34 human NB tumor samples.