The consistency of predicted R<sub>1</sub>, R<sub>1</sub>-2 and D-1 in WD40 blades of Met30p and MDV1p with the mutagenesis studies.

<p>The blue-colored residues are in the predicted DHSW tetrad. The green-colored residues are in the predicted WD<sub>b–a</sub> and WD<sub>c–d</sub> bulges. The red-colored residues were studied by the mutagenesis. The last WD40 blade is composed of strand d at the beginning of WD40 domain and the last strand a, b and c.</p>