The <i>bona fide</i> DNA-binding domain of Sp2 is dispensable for genomic binding.

<p><i>Sp2ko</i> MEFs re-expressing Flag-tagged full-length Sp2 (Flag-Sp2FL), the N-terminal domain (Flag-Sp2NT) or the C-terminal zinc finger domains (Flag-Sp2ZF) were subjected to ChIP-seq analysis. (A) Venn diagram showing the overlap of sites bound by Flag-Sp2FL, Flag-Sp2NT and Flag-Sp2ZF with sites bound by endogenous Sp2 in wild type MEFs. (B) Full-length Sp2 and the N-terminal region of Sp2 have similar chromatin binding efficiencies. ChIP-seq tag counts (normalized to 20x10<sup>6</sup> reads) at individual Flag-Sp2NT and Flag-Sp2FL peaks found in both samples were plotted against each other. (C) Sites bound by Flag-Sp2NT represent high affinity binding sites of native Sp2. Individual native Sp2 peaks [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005102#pgen.1005102.ref018" target="_blank">18</a>] were plotted against their normalized tag counts. Those sites that were also detected by ChIP-seq in <i>Sp2ko</i> MEFs expressing the N-terminal domain of Sp2 (Flag-Sp2NT mutant) were overlaid with red dots. (D) Representative binding profiles of Sp2 in wild type MEFs (Sp2 / wt), and of Flag-Sp2FL, Flag-Sp2NT and Flag-Sp2ZF expressed in <i>Sp2ko</i> MEFs. (E) Schematic representation of the genomic binding features of Sp1/Sp3 and Sp2 based on the results shown in Figs. <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005102#pgen.1005102.g001" target="_blank">1</a>–<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005102#pgen.1005102.g004" target="_blank">4</a>.</p>