The analysis of liver glycogen by PAS staining.

A-C: Liver glycogen as demonstrated by PAS staining (purple-red, 100×). A: Liver glycogen in the control group: large areas of hepatic cells around the central vein with positive PAS staining. B: Liver glycogen in the TS group: PAS-positive cells were significantly reduced, mainly at the edge of the portal area, with only scattered, sporadic purple liver cells surrounding the central vein. C: Liver glycogen in the TS&RT group: PAS-positive cells remained mainly in the area around the portal vein, and the number of PAS-positive cells increased around the central vein. D: A semi-quantitative analysis of liver glucose in the PAS-stained slides confirmed the results obtained for the serum glucose levels. Each value represents the percentage of the mean area of glycogen density in each group. n = 150 fields (10 rats) for each value. (Data are expressed as the mean ± SD. ** P <0.01, significantly different from control group.).