The PE domain contributes to LipY aggregation in vitro.

LipYΔPE and the PE domain were resolved on a Superdex 200 gel filtration column. Both proteins were pre-incubated with a 270 fold molar excess of Tween 20 for 1 hour prior to being resolved on the column. In the gray PE domain trace, Peak 1 (~9.5 mL) elutes near the void volume and Peak 2 (~13 mL) elutes in the included volume. In the black LipYΔPE trace, a weak A₂₈₀ signal was observed between 9 and 12 mL and a single distinct peak (Peak 2) elutes in the included volume. Western blots against the His tag of each construct show that the majority of the PE domain elutes in the void volume while the majority of LipYΔPE elutes in the included volume.