Fig_5.tif (1.32 MB)
The GFP-ML1Nx2 probe labels punctate structures even in the absence of PIKfyve.
figure
posted on 2015-10-13, 03:18 authored by Gerald R. V. Hammond, Shunsuke Takasuga, Takehiko Sasaki, Tamas BallaImages show a representative wild-type and PIKfyve knock-out (KO) ES cells expressing the GFP-ML1Nx2 probe. Note the fluorescent puncta in the PIKfyve KO cell which showed swollen endosomes, a hallmark of PtdIns(3,5)P2 deficiency. Data are representative of 11 cells, scale bar = 15 μm.
