TMEM45A is not essential for the impermeability of the cornified layer.
(A) Toluidine blue exclusion assay in E16.5 and P1 of WT, KO and HET mouse littermates. (B-C) Permeability of the cornified layer of human epidermis reconstructed from keratinocytes transduced with NT or TMEM45A shRNA to Lucifer Yellow, tested after 7 or 11 days of reconstruction (n = 1). (B) After the incubation, the fluorescence in medium was quantified. (C) Tissues were processed and analyzed using fluorescence microscopy. The dotted lines delineate the polycarbonate filter (scale bars: 50 μm).