Fig_7.tif (1.08 MB)
TMEM45A is not essential for the impermeability of the cornified layer.
figure
posted on 2016-01-28, 12:37 authored by Aurélie Hayez, Edith Roegiers, Jérémy Malaisse, Benoit Balau, Christiane Sterpin, Younes Achouri, Catherine Lambert De Rouvroit, Yves Poumay, Carine Michiels, Olivier De Backer(A) Toluidine blue exclusion assay in E16.5 and P1 of WT, KO and HET mouse littermates. (B-C) Permeability of the cornified layer of human epidermis reconstructed from keratinocytes transduced with NT or TMEM45A shRNA to Lucifer Yellow, tested after 7 or 11 days of reconstruction (n = 1). (B) After the incubation, the fluorescence in medium was quantified. (C) Tissues were processed and analyzed using fluorescence microscopy. The dotted lines delineate the polycarbonate filter (scale bars: 50 μm).
