TCDD causes apoptosis of Foxp3<sup>-</sup>CD4<sup>+</sup> T cells but not Tregs.

<p>Foxp3-GFP mice were HSV-1 infected and sacrificed on day 8 pi. A, Induction of <i>in vitro</i> apoptosis by different concentrations of TCDD on Foxp3<sup>-</sup>CD4<sup>+</sup> T cells but not on Foxp3<sup>+</sup>CD4<sup>+</sup> T cells. Cells isolated from spleens and DLNs of HSV-1 infected mice were cultured <i>ex vivo</i> in the presence of RPMI or RPMI with different concentrations of TCDD for 5 hours and thereafter stained for Annexin-V, representative FACS plots showing Annexin-V expression on gated CD4<sup>+</sup> T cells. B, Representative FACS plots of Foxp3<sup>+</sup>CD4<sup>+</sup> T cells in splenocytes and DLN cells from HSV-1 infected mice treated <i>in vitro</i> with different concentrations of TCDD for 5 hours. C, Bar diagram showing the percentages of Annexin V<sup>+</sup>Foxp3<sup>-</sup>CD4<sup>+</sup>, Annexin V<sup>+</sup>Foxp3<sup>+</sup>CD4<sup>+</sup> and Foxp3<sup>+</sup>CD4<sup>+</sup> under the indicated conditions in the DLN is shown. Data are representative of 3 independent experiments. Data were analyzed using one-way ANOVA test with Dunett's post hoc test settings and show mean values ± SEM (n = 12). <i>P</i>≤0.001(***), <i>P</i>≤0.01(**), <i>P</i>≤0.05(*).</p>