Figure_4.tif (1.91 MB)
Systemic modulation of regulatory components by HDAC2 in G1/S cell cycle transition.
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posted on 2011-11-23, 00:02 authored by Ji Heon Noh, Kwang Hwa Jung, Jeong Kyu Kim, Jung Woo Eun, Hyun Jin Bae, Hong Jian Xie, Young Gyoon Chang, Min Gyu Kim, Won Sang Park, Jung Young Lee, Suk Woo NamHep3B cells were transfected with no siRNA (None), oligofectamine only (Reag), 100 nmol/L scrambled siRNA (Scr) or 100 nmol/L of two different HDAC2 specific siRNAs (#1 and #2), and were harvested at 72 h post-transfection. (A) Immunoblotting of CDKs, cyclins and CDK inhibitors of G1/S transition were performed. (B) Effects of HDAC2 depletion on pRb and E2F/DP1 target gene expressions. (C) Protein expressions of p21WAF1/Cip1, CDK2 and PCNA in human HCC tissues were analyzed by immunoblotting. All experiments were repeated three times with same results.
