Figure_4.tif (3.13 MB)

Systemic administration of IL-10 diminishes SPX-induced fat accumulation, infiltration of macrophages, and pro-inflammatory responses in the islets.

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posted on 2013-02-19, 17:52 authored by Koro Gotoh, Megumi Inoue, Kentaro Shiraishi, Takayuki Masaki, Seiichi Chiba, Kimihiko Mitsutomi, Takanobu Shimasaki, Hisae Ando, Kansuke Fujiwara, Isao Katsuragi, Tetsuya Kakuma, Masataka Seike, Toshiie Sakata, Hironobu Yoshimatsu

(A) Representative images of CD11c staining (upper sections) and CD206 staining (lower sections) in intra-islet areas in pancreas sections from each group. Scale bar = 100 µm. (B−D) CD11c-positive areas (B), CD206-positive areas (C), and M1/M2 ratios (D) in the islets in each group (n = 6). *P<0.05 vs. the Standard-Sham group, ^#P<0.05 vs. the HF-Sham group. (E) Serum levels of IL-1β, MCP-1 and IL-10 in sham and SPX mice fed standard chow or a HF diet. *P<0.05 vs. the Standard-Sham group, ^#P<0.05 vs. the Standard-SPX group. (F) Serum cytokine changes relative to the Standard-Sham group. *P<0.05 vs. IL-1β and MCP-1. (G) Serum leptin levels in each group (n = 6). *P<0.05 vs. the Standard-Sham group, ^#P<0.05 vs. the HF-Sham group, ^$P<0.05 vs. the HF-SPX group. Treatment groups: Standard-Sham, fed standard chow, given serum albumin, and sham-operated; HF-Sham, fed a HF diet, administered serum albumin, and sham-operated; HF-SPX, fed a HF diet, given serum albumin, and splenectomized; HF-SPX+IL-10, fed a HF diet, given IL-10, and splenectomized; Pair-fed, administered serum albumin, sham-operated, and fed the same amount of food as that consumed by the HF-SPX group.