Swimming training cannot attenuate ISO-induced cardiac fibrosis in AMPKα2^-/- mice.

(A) Representative micrographs of Sirius red-stained sections of the left ventricle (LV) (bar = 400 μm). (B) Quantification of mean cardiac interstitial collagen content from Sirius red-stained sections (n = 8). RT-PCR analysis of mRNA expression of collagen I (C), collagen III (D), and CTGF (E) normalized to that of GAPDH (all n = 5). ^*P<0.05, ^***P<0.001 sed+ISO vs. sed+vehicle; NS, not significant. Data are mean±SEM.