Fig_4.tif (1.27 MB)

Swimming training attenuated ISO-induced reactive oxygen species (ROS) production in AMPKα2^+/+ but not AMPKα2^-/- mice.

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posted on 2015-06-12, 02:46 authored by Xiaowei Ma, Yongnan Fu, Han Xiao, Yao Song, Ruifei Chen, Jing Shen, Xiangbo An, Qiang Shen, Zijian Li, Youyi Zhang

(A) Fluorescent microscopy of representative DHE staining for ROS level in LV sections for AMPKα2^+/+ mice (bar = 100 μm). (B) Quantification of fluorescence intensity of DHE in AMPKα2^+/+ sections (n = 6). (C) Malonaldehyde (MDA) content in myocardial tissue in AMPKα2^+/+ mice (n = 6). ^**P<0.01, ^***P<0.001 sed+ISO vs. sed+vehicle; ^#P<0.05 swim+ISO vs. sed+ISO. (D) Fluorescent microscopy of representative DHE staining for ROS level in LV sections for AMPKα2^-/- mice (bar = 100 μm). (E) Quantification of fluorescence intensity of DHE in AMPKα2^-/- sections (n = 6). (F) MDA content in myocardial tissue in AMPKα2^-/- mice (n = 6). ^**P<0.01, ^***P<0.001 sed+ISO vs. sed+vehicle; NS, not significant. Data are mean±SEM.