Swimming training attenuated ISO-induced NADPH oxidase expression in AMPKα2^+/+ mice but not AMPKα2^-/- mice.

(A) Western blot analysis of myocardial protein levels of NOX4 and eIF5 for AMPKα2^+/+ mice. (B) Quantification of NOX4 relative to eIF5 for AMPKα2^+/+ mice (n = 4). (C) RT-PCR analysis of mRNA expression of NOX4 normalized to that of GAPDH for AMPKα2^+/+ mice (both n = 5). ^**P<0.01, ^***P<0.001 sed+ISO vs. sed+vehicle; ^#P<0.05, ^##P<0.01 swim+ISO vs. sed+ISO. (D) Western blot analysis of myocardial protein levels of NOX4 and eIF5 for AMPKα2^-/- mice. (E) Quantification of NOX4 relative to eIF5 in AMPKα2^-/- mice (n = 4). (F) RT-PCR analysis of mRNA expression of NOX4 normalized to that of GAPDH for AMPKα2^-/- mice (n = 6). ^***P<0.001 sed+ISO vs. sed+vehicle; NS, not significant. Data are mean±SEM.