Swimming training activated adenosine monophosphate-activated protein kinase (AMPK) in AMPKα2^+/+ mouse hearts but not AMPKα2^-/- mice.

(A) Western blot analysis of protein levels of phosphorylated and total AMPK at different swimming times in AMPKα2^+/+ mice. (B) Quantification of phosphorylated AMPK relative to total AMPK (all n = 4). ^*P<0.05 swim vs. sed 2 weeks after swimming; ^#P<0.05 swim vs. sed 4 weeks after swimming. (C) Western blot analysis of phosphorylated AMPK, total AMPKα, AMPKα2 and eukaryotic translation initiation factor 5 (eIF5) at the end of swimming training in AMPKα2^+/+ and AMPKα2^-/- mice. (D) Quantification of phosphorylated AMPK relative to eIF5 (n = 6). ^***P<0.001. Data are mean±SEM.