Summary of genotyping results.

<p>(A) Representative data obtained from genotyping of wild-type (+/+), heterozygous (+/−) and homozygous (−/−) fishes was validated by polymerase chain reaction. (B) Brain tissues were analyzed by western blot using an FMRP specific antibody. Lane 1 contains wild-type (WT) and Lane 2 contains <i>fmr1−/−</i> (KO). The arrow points at FMRP located. The FMRP protein is completely absent in <i>fmr1−/−</i>.</p>