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Sub-cellular localization patterns for some category 1, 2, 3 and 4 GFP-tagged proteins.

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posted on 2011-05-17, 01:30 authored by Barbara Meissner, Teresa Rogalski, Ryan Viveiros, Adam Warner, Lorena Plastino, Adam Lorch, Laure Granger, Laurent Segalat, Donald G. Moerman

Panels A and B show single symmetrical, spindle–shaped body wall muscle cells exhibiting GFP-tagged protein localization. Panels C, D and E show parts of two adjacent body wall muscle cells exhibiting GFP-tagged protein localization. The category 1 proteins, F15G9.1 (A) and B0303.2 (B), are localized to the myofilament lattice and the dense bodies. The category 2 protein, M01E11.7 (C), is found in the dense bodies, M-lines and cell attachment sites. The category 3 protein, M03A8.4 (D), is a component of the dense bodies and cell attachment sites and the category 4 protein, T04C9.4 (E), is localized to the dense bodies and cytoplasm. The thin arrows point to dense bodies, the thick arrows to the cell attachment sites and the closed arrow head points to the M-line. The asterisk indicates thick filaments and the open arrowhead indicates thin filaments. Bars represent 10 µm.

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