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Structured sampling schemes.
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posted on 2014-03-03, 02:55 authored by Ramona L. Walls, John Deck, Robert Guralnick, Steve Baskauf, Reed Beaman, Stanley Blum, Shawn Bowers, Pier Luigi Buttigieg, Neil Davies, Dag Endresen, Maria Alejandra Gandolfo, Robert Hanner, Alyssa Janning, Leonard Krishtalka, Andréa Matsunaga, Peter Midford, Norman Morrison, Éamonn Ó. Tuama, Mark Schildhauer, Barry Smith, Brian J. Stucky, Andrea Thomer, John Wieczorek, Jamie Whitacre, John Wooley(A) Biological sampling can be structured in both space and time. Environmental sampling of ocean water often includes sampling along a transect, with samples collected at multiple depths at each location. Additionally, each sample of water collected may be subsampled for metagenomic analysis or measuring chemical content. (B) Sampling schemes in ecological studies are often nested and may include plot; subplot or transect within plot; individual within plot, subplot, or transect; organ (e.g., leaf) within individual; tissue within organ; and DNA or mineral (e.g., C or N) within tissue. DNA extracted from a leaf of a tree that is present in a sub-plot may therefore be characterized by environmental features of the plot.
