Fig_6.tif (3.14 MB)
Structural characterization of avian H5N1 rNA using TEM with single particles reconstruction.
figure
posted on 2015-08-17, 02:58 authored by Marua Prevato, Ilaria Ferlenghi, Alessandra Bonci, Yasushi Uematsu, Giulia Anselmi, Fabiola Giusti, Sylvie Bertholet, Francois Legay, John Laird Telford, Ethan C. Settembre, Domenico Maione, Roberta Cozzi(A) Typical negative staining TEM image of avian H5N1 rNA. Scale bar corresponds to 200 nm. (B) Representative class averages of avian H5N1 rNa tetramers. Each class average (~50 images per class) contains particles selected from several micrographs and represents the different orientations of the enzyme. Scale bar corresponds to 10 nm. (C) Top (upper panel) and side (lower panel) surface views of the reconstructed avian H5N1 rNA globular head obtained at a resolution of 24 Å (FSC = 0.5).
