Stereomicroscopic view of cultured adult mouse testis tissues.

(A) Six-week-old Acr-GFP mouse testis tissue fragments on culture days 0, 3, and 10. Upper row is GFP excitation view and lower row is bright view. (B) Six-week-old Acr-GFP mouse testis tissue fragments on culture days 0, 15, and 82. Weak GFP expression was maintained. (C-E) Immunohistochemistry of cultured tissues with antibodies to BrdU and GFP, counterstained with Hoechst. Testis tissue fragments of 6-week-old Acr-GFP (C and D) and 24-week-old Gsg2-GFP (E) mice were cultured, and then analyzed on days 42 (C), 85 (D), and 48 (E), respectively. The dashed rectangular area in the left picture of C is enlarged on the right. Arrows indicate BrdU-positive round spermatids in C and elongating spermatids in E. Scale bars, 1 mm (A, B); 10 μm (C, E); 50 μm (D).