Stat92E sensor activity in ept mutant eye-antennal cells.

Eye discs carrying clones of ept2 mutant cells (A-A″) or FRT80B control cells (B-B″) marked by the absence of GFP (green) and stained for expression of β-galactosidase (β-gal; red in A′ and B′) to detect expression of the 3xGAS-lacZ Stat-reporter. Inset in (A-A″) shows an ept2 mutant clone that shows cell autonomous activation of 3xGAS-lacZ. Because the 3xGAS-lacZ transgene and the ept gene are both located on chromosome arm 3L, the reporter is present in two copies in both FRT80B and ept mutant clones; images in A′ and B′ were captured using exactly the same optical settings. (C–E) Expression of the 10xStat>GFP transgene (green) in eptX1,H99 mosaic discs in which mutant cells are marked by the absence of β-gal (red). Arrowhead marks clone of eptX1,H99 mutant cells in the eye disc that do not activate 10xStat>GFP; arrow marks an example of an antennal clone that activates 10xStat>GFP within the clone and in surrounding wild type cells. Images in (E-E″) are of the antennal region of an eptX1,H99 mosaic disc. White outlines in panel (E″) denotes boundaries of GFP-expressing cells. Expression of the Stat reporter 10xStat>GFP (green) in a control FRT80B/M(3) eye disc (F) and an ept2 eye-antennal tumor (G). The disc in (F) was imaged at half the fluorescence intensity relative to the control disc in (G).