Standardization of microparticle (MP) enumeration. ISTH-“Vascular Biology Workshop”.
1st Graph is a graph of forward scatter (FSC-A) vs. side scatter (SSC-A) parameters showing a blend of monodisperse fluorescent beads (Megamix) of three diameters (0.5, 0.9 and 3 µm). 2nd Graph defines the MP gate which includes all events of 0.9 µm and below (microparticles) and excludes all above (cells). 3rd Graph locates the position of 3 µm beads so as to position the 10 µm counting beads 1 log ahead.