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Stable transfection and colony selection.

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posted on 2012-11-19, 00:15 authored by Lei Yu, Chunming Rao, Xinchang Shi, Yonghong Li, Kai Gao, Xuguang Li, Junzhi Wang

GCA expression in 293GCAC3. The expression of DDK-tagged GCA in 293GCAC3 and parental HEK293 were analyzed by indirect immunofluorescence staining and flow cytometry. The results indicated that almost all of the 293GCAC3 cells over-expressed DDK-tagged GCA.

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    transfection

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