Figure_4.tif (1.24 MB)
Spread colony phenotype of 3610 spontaneous mutants.
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posted on 2012-11-02, 02:45 authored by Olga Zafra, María Lamprecht-Grandío, Carolina González de Figueras, José Eduardo González-PastorA. Differences in colony morphology between 3610 (left) and SPR, a spontaneous spread mutant (GP305, right) growing on LB. The picture in the middle corresponds to a 3610 colony with two branches of spread mutants (arrow). Bar represents 1 cm. B. eDNA production of wild type (3610) and several isogenic spread mutants: SPR-1 (GP305), SPR-2 (GP306), SPR-3 (GP307) and SPR-4 (GP319). Strains were grown in MSgg at 37°C with aeration (growth was similar for all the strains). eDNA refers to the eDNA concentration in the culture supernatant. Data presented are representative of results obtained in two independent experiments.