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Spin labeling of Ure2p1–89-M.

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posted on 2012-10-16, 02:17 authored by Sam Ngo, Vicky Chiang, Elaine Ho, Linh Le, Zhefeng Guo

(A) Sequence of Ure2p prion domain construct with positions for spin labeling indicated by arrow heads. The amino acid sequences of Ure2p prion domain, Sup35p M domain, and other linker/tag regions are shown in black, blue, and green, respectively. (B) A stick model of spin label R1 in the crystal structure of spin-labeled T4 lysozyme (PDB entry 2IGC).

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