Specificity of the anti-STIP1 antibody (panels A–D) and the intensity of immunostaining for STIP1 (panels E–G).

STIP1 expressed in the cytoplasm of ovarian cancer cells is stained brown. The immunoreactivity of STIP1 to the anti-STIP1 antibody in cancer tissues (A, C) was abrogated by the addition of 15 µg of recombinant human STIP1 during incubation (B, D); these results support the specificity of the anti-STIP1 antibody. The intensity of the STIP1 staining was graded as 1 (E), 2 (F), and 3 (G). All of the slides presented here were from ovarian serous carcinomas. The scale bars represent 100 µm (A, B) or 20 µm (C–G).