Specificity analysis of IQ Plus™ WSSV Kit with POCKIT System using WSSV, IHHNV, MBV, and HPV-positive samples.

Genomic DNA extracts of diseased and healthy shrimp samples were subjected to analysis by IQ2000™ WSSV (A), IHHNV (B), MBV (C), and HPV (D) DPS assays, as well said Plus™ WSSV Kit with POCKIT system. Positive standard DNA provided by the kit was serially diluted (10³, 10² and 10¹ copies) as suggested by the manufacturer. Nested PCR amplicons were analyzed by agarose gel electrophoresis and ethidium bromide staining. The kits were designed to generate three amplicons (arrows) from target viral genome. The number of the amplicon bands positively correlates with the concentrations of the starting DNA template. The same set of samples was analyzed by IQ Plus™ WSSV Kit with POCKIT System analysis (E). M, DNA weight molecular markers (bp); N, negative control (ddH₂O); Std, standard plasmid.