Specification and differentiation of the ocular adnexa at E15.5.

Cryosections were stained with various markers of keratinocyte differentiation. For orientation purposes c  =  cornea and r  =  retina in all images. Keratin 10 (K10) is expressed in papebral epidermis (ep) in wild type (A, +/+) and LIMK2-deficient (B, −/−) eyelids. The conjunctiva (conj.) is negative for K10 expression. An antibody recognizing phosphorylated c-Jun labels nuclei in cells (arrows) at the tip of the eyelid in both +/+ (C) and −/− (D) mice. Keratin 6 (K6) is expressed in epithelial cells at the tip of the eyelids in both +/+ (E) and −/− (F) mice, consistent with induction of a migratory phenotype. Scale bar  = 100 μm.