Simvastatin and benznidazole decreased cell adhesion molecule amount during Trypanosoma cruzi infection of endothelial cells.

Endothelial-like (EA.hy926) and HUVEC cells were incubated with 5 μM simvastatin or 20 μM benznidazole 0.025% v/v final concentration. After 24 hours, the medium was replaced, and the cells were infected at a MOI of 10. After 16 hours of infection, cells were harvested, and total protein expression was determined by Western blot. The data are presented as total of E-Selectin (A), VCAM-1 (B) and ICAM-1 (C) content. Upper panels correspond to representative blot images. Bottom panels correspond to relative blot quantification using β-tubulin as a control. Controls were incubated with DMSO vehicle alone. The data are expressed as the mean ± SD from three independent experiments. One-way ANOVA and Tukey’s post-test analysis were used to assess significant differences. ‡ p<0.001 vs. control; * p<0.05; *** p< 0.001 vs. T. cruzi.