Sequence variability in LRRNT and LRRCT.

<p>The Shannon entropy was calculated from 192 sea lamprey (<i>Petromyzon marinus</i>) VLRA sequences (A), 640 sea lamprey (<i>Petromyzon marinus</i>) VLRB sequences (<i>B</i>), 102 Japanese lamprey (<i>Lethenteron japonicum</i>) VLRC sequences (<i>C</i>), 98 inshore hagfish (<i>Eptatretus burgeri</i>) VLRB sequences (<i>D</i>), or 226 inshore hagfish (<i>Eptatretus burgeri</i>) VLRC sequences (previously reported as hagfish VLRA) (<i>E</i>). The molecular surfaces were calculated with the structure of lamprey VLRA.R2.1 (PDBID 3G3A) (<i>A</i>), lamprey VLRB.2D (PDBID 3M18) (<i>B</i>), lamprey VLRC from this study (<i>C</i>), hagfish VLRB.59 (PDBID 2O6S) (<i>D</i>), and hagfish VLRA.29 (VLRC.29 according to the new nomenclature) (PDBID 2O6Q) (<i>E</i>). Sequence variability is indicated by the color gradation from red (the most variable positions) to blue (the least variable positions) as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085875#pone-0085875-g003" target="_blank">Figure 3</a>. LRR1, LRRVs, LRRVe and CP are shown in gray.</p>