Sensitivity of the Total DnaK to the Change in the Transcription Rate Constant for DnaK at Low Temperature

<div><p>The plot reports the sensitivity computed for the parameters values that satisfy the required yield and efficiency (yield > 0.9 and efficiency > 0.85 at low and high temperature). Yield and efficiency were computed by searching the 2-D parameter space consisting of the transcription rate constant for σ<sup>32</sup> and the association constant between σ<sup>32</sup> and DnaK as follows: </p><p></p>for the various systems, <p></p><p>(A) DEG-FB/FF double mutant (SEQ-FB system): <i>K[5]</i> = <i>K[6]</i> = 0 M<sup>−1</sup>, <i>kx[1]</i> = <i>kx[2]</i> = 0 min<sup>−1</sup>, <i>η</i> = 1,</p><p>(B) FF mutant (SEQ-FB + DEG-FB system): <i>K[5]</i> = <i>K[6]</i> = 1 × 10<sup>8</sup> M<sup>−1</sup>, <i>kx[1]</i> = <i>kx[2]</i> = 5 min<sup>−1</sup>, <i>η</i> = 1,</p><p>(C) Wild-type (SEQ-FB +DEG-FB + FF system): <i>K[5]</i> = <i>K[6]</i> = 1 × 10<sup>8</sup> M<sup>−1</sup>, <i>kx[1]</i> = <i>kx[2]</i> = 5 min<sup>−1</sup>, <i>η</i> = 1 (low), <i>η</i> = 2 (high).</p></div>