Senescence quantification in PDMSCs isolated from normal and preeclamptic placentae.

(A) Normal (left panel) and PE (right panels) PDMSCs representative images after SAβ-gal nuclear staining. EB: Embryoid Body. White Arrows indicate blu nuclei positive for SAβ-gal staining, indicative of cellular senescence. (B) Quantitative analysis of cellular senescence in Normal and PE-PDMSCs. For quantitative analysis, SAβ-gal positive cells were considered to be senescent cells. The percentages of senescent cells after 48 h of incubation were determined in Normal and PE-PDMSCs populations. At least 200 cells were counted in each group. Results are expressed as means ± SE of six independent samples. Statistical significance (*) has been considered as p<0.05.