Secretion of TGFβig-h3 is stimulated by chemerin and IGF.

A. Western blot analysis of TGFβig-h3 in media from MSC cells shows stimulation by chemerin and IGF-II and inhibition by CCX832 and AG1042, respectively. B. Stimulated secretion of TGFβig-h3 is maintained after cycloheximide treatment. TGFβig-h3 abundance in cell extracts was unchanged (middle panel); GAPDH was used as a loading control for the cell extracts (bottom panel). C. The calcium ionophore, ionomycin (1μM) stimulated TGFβig-h3 secretion comparable to IGF-I (50ng.ml-1) and IGF-II (100ng.ml-1). D. In calcium-free medium stimulated secretion in response to IGF-II is inhibited.