Figure_2.tif (1.44 MB)
Schematic representation of the E. coli-bifidobacterial shuttle vectors pDM1 and pDM2.
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posted on 2014-04-17, 04:13 authored by Mary O′Connell Motherway, Debbie Watson, Francesca Bottacini, Tyson A. Clark, Richard J. Roberts, Jonas Korlach, Peggy Garault, Christian Chervaux, Johan E. T. van Hylckama Vlieg, Tamara Smokvina, Douwe van SinderenpDM1 is a derivative of pAM5 where the tetracycline resistance gene has been replaced with the spectinomycin resistance cassette. pDM2 is a derivative of the plasmid pDG7 where the spectinomycin resistance cassette was cloned in the unique EcoR1 and HindIII sites of pDG7.