Fig_3.tif (270.19 kB)
Schematic diagram of the firefly/renilla duo-luciferase reporter vector.
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posted on 2015-04-22, 03:40 authored by Jane Yi Lin Tan, Nagy A. Habib, York Wieo Chuah, Yin Hoe Yau, Susana Geifman-Shochat, Wei Ning ChenThe 3’UTRs of CDKN1β and E2F7 are cloned downstream of the firefly luciferase reporter gene, while a random sequence is cloned in the case of the control vector. Renilla luciferase activity is used as the internal control, and the firefly luciferase activity is normalized against that measured of renilla luciferase.
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target protein expression levelsbioinformatics analysissprFunctional analysishccNew Approachluciferase activityCDKNCell proliferationHepG 2 cellsHepatocellular carcinomamirnaAberrant levelssurface plasmon resonancebindingmirMicroRNAs MicroRNAsvivo luciferase assayutrmRNA targetsmapktranscriptional factor E 2F
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