SalA alleviates impairment of endothelial NO formation in response to IS/RP.

2013-02-20T01:47:56Z (GMT) by Dan Yang Ping Xie Zhihua Liu
<p>HUVECs were pretreated with SalA for 30 min in various concentrations, and then subjected to IS/RP treatment. (a) The MKP-3 and eNOS mRNA levels in each group were detected by quantitative RT-PCR (n = 5, means±SEM). *<i>P</i><0.05 vs. control group; <sup>#</sup><i>P</i><0.05 vs. non-SalA-treated IS/RP group. (b) The protein levels of MKP-3, ERK1/2, eNOS, and GAPDH were detected by immunoblot analysis. A representative blot was shown (n = 4). (c) The NOS activity in the cell lysates was determined via examining the efficiency in conversion of [3H]L-arginine to [3H]L-citrulline (n = 4, means±SEM). *<i>P</i><0.05 vs. control group; <sup>#</sup><i>P</i><0.05 vs. non-SalA-treated IS/RP group. (d) NO formation was detected as nitrite released into supernatant of confluent cultures of HUVECs (n = 3, means±SEM). *<i>P</i><0.05 vs. control group; <sup>#</sup><i>P</i><0.05 vs. non-SalA-treated IS/RP group. (e) Cell apoptosis was determined by TUNEL assay. The number of TUNEL-positive cells was quantified, and at least 120 cells per dish were counted (n = 3, means ± SEM.). *<i>P</i><0.05 vs. control group; <sup>#</sup><i>P</i><0.05 vs. non-SalA-treated IS/RP group.</p>