Figure 2.TIF (1.1 MB)
S. Typhimurium NSS does neither require adhesins nor chemotaxis.
figure
posted on 2012-07-26, 01:02 authored by Benjamin Misselwitz, Naomi Barrett, Saskia Kreibich, Pascale Vonaesch, Daniel Andritschke, Samuel Rout, Kerstin Weidner, Milos Sormaz, Pascal Songhet, Peter Horvath, Mamta Chabria, Viola Vogel, Doris M. Spori, Patrick Jenny, Wolf-Dietrich HardtQuantitiative analysis of the initial host-cell interaction byS.Tm-T1(pGFP), S.Tm-T1-Fi(pGFP) and S.TmΔcheY(pGFP), lacking either the TTSS-1 apparatus,TTSS-1 and type 1 fimbriae or the four effectors SopE, SopE2, SopB and SipA as well as the protein CheY, essential for chemotaxis. (A), (C), (E)Different stages of NSS were quantified as described for Fig. 1D.The following numbers of bacteria were analyzed: S.Tm-T1(pGFP), n = 114; S.Tm-T1-Fi(pGFP),n = 91;S.TmΔcheY(pGFP), n = 39.Time-lapse microscopy data was derived from 2 to5independent experiments. (B), (D), (F) Numbers of stops by an individual bacterium were quantified as described in Fig. 1E.
