SEC elution profiles of cross-linked and intact α-crystallin on a TSK-gel HW-55f column.

<p>The fraction of cross-linked protein marked with gray color was isolated for further testing the chaperone-like activity. Triangles point out retention time of the protein standards: thyroglobulin (660 kDa), catalase (440 kDa), aldolase (158 kDa), BSA (67 kDa), α-crystallin (20 kDa).</p>