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SDS-PAGE of purified membrane protein by Ni-affinity chromatography in different imidazole concentration.

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posted on 2015-09-30, 03:01 authored by Jian Wang, Yongxin She, Miao Wang, Maojun Jin, Yongfei Li, Jing Wang, Yuan Liu

The imidazole concentration in the elution buffer was optimized at 3 levels: 75 (A), 125 (B), and 250 Mm (C). When it reached 250 mM, the eluate in 7-10 tubes contained abundant recombinant β₂-AR protein.

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Several parameters Receptor Assay swine urine elra crude membrane protein HEK 293 cells agonist expression system receptor IC 50 concentrations kidney cells adrenergic activity identification Multiresidue Method recovery rates ar β2 Adrenergic Receptor animal urine β Agonists

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