SDS-PAGE, immunoblot analyses, Nanoparticle tracking analysis and electron microscopic analyses of OMV samples from the wild type strain C6706 and the <i>prtV</i> mutant.

<p>(A) SDS-PAGE and Coomassie blue staining of OMVs samples from <i>V</i>. <i>cholerae</i> wild type C6706 (lane 1) and its derivative <i>prtV</i> mutant (lane 2). (B) Immunoblot analysis of PrtV protein in the OMV samples from the wild type strain C6706 (upper panel, lane 1) and the <i>prtV</i> mutant (upper panel, lane 2) using anti-PrtV polyclonal antiserum. Immunoblot analysis of OMV samples using anti-OmpU antiserum as a OMV marker (middle panel) and anti-Crp polyclonal antiserum as a cytoplasmic protein marker (lower panel). (C) Nanoparticle tracking analysis measurement of OMVs isolated from the wild type <i>V</i>. <i>cholerae</i> strain C6706 showing the sizes and total concentration of OMVs. (D) Nanoparticle tracking analysis measurement of OMVs isolated from the Δ<i>prtV</i> mutant showing the sizes and total concentration of OMVs. (E) Electron microscopy of OMVs from the wild type <i>V</i>. <i>cholerae</i> strain C6706 (a) and the <i>prtV</i> mutant (b). Immunogold labeling of OMVs from <i>V</i>. <i>cholerae</i> wild type strain C6706 (c) and the <i>prtV</i> mutant (d). White arrow points to gold particles associated with OMVs. Bars; 150 nm.</p>