Figure_6.tif (896.99 kB)

SDS-PAGE and western blot analyses of the purified sTNFRII-gAD.

figure

posted on 2014-10-23, 17:05 authored by Qinzhen Cai, Ai Zhao, Yuting Yin, Lisha Ma, Zhenzhen Jiao, Huilin Zhi, Shouhua Lai, Sha Cheng, Hongmei Yang, Yinxiang Lu, Katherine A. Siminovitch, Jimin Gao

(A) SDS-PAGE analysis of sTNFRII-gAD purified by anion exchange chromatography. Lane 1:molecular weight markers (kDa); lanes 2, 3: The loaded protein samples were about 5.0 µg each loading in non-reducing and reducing conditions, respectively. (B) SDS-PAGE analysis of sTNFRII-gAD separated by HiLoad 16/60 Superdex 200 chromatography. Lane 1, protein molecular weight markers; Lane 2, multimeric sTNFRII-gAD (peak 1); Lane 3, trimeric sTNFRII-gAD (peak 2); Lane 4, monomeric sTNFRII-gAD (peak 3). The loaded protein samples were about 2.0, 5.0, and 3.0 µg, respectively. (C) Western blot analysis of purified trimeric sTNFRII-gAD under non-reducing/reducing conditions. Lane 1, molecular weight markers; Lanes 2 and 3 The loaded protein samples were about 5.0 µg and 3.0 µg in non-reducing and reducing conditions, respectively.