Resveratrol inhibits the Wnt/β-catenin signaling through regulating MALAT1.

(A): Resveratrol negated LiCl induced nuclear translocation of β-catenin. Immunofluorescence staining of β-catenin in LoVo cells treated with LiCl, with or without resveratrol. (B): Nuclear extracts from LoVo cells expressing MALAT1-shRNA, MALAT1-overexpressed, empty vector, treated with LiCl with or without resveratrol, were probed for β-catenin, c-Myc, MMP-7. (C): Quantification of β-catenin, c-Myc, MMP-7 protein levels from the data shown in (A). **p<0.01, compared with blank group or LiCl alone group. (D): The relative LEF/TCF promoter activities in LoVo cells expressing MALAT1-shRNA, MALAT1-overexpressed, empty vector, treated by LiCl, with or without resveratrol. **p<0.01, compared with blank group or LiCl alone group. (E): No interaction between MALAT1 and β-catenin. Insert: RT-PCR result showing amount of RNA pulled-down by RNA-binding protein immunoprecipitation. As negative controls, no cDNA (blank) or IgG alone was used. Lower pane: quantification result of the insert.