Results from CART analysis of phytoplankton production rates (mgC m<sup>−3</sup> h<sup>−1</sup>)

2013-09-11T00:00:00Z (GMT) by Brian K Sorrell Ian Hawes Karl Safi
<p><strong>Figure 3.</strong> Results from CART analysis of phytoplankton production rates (mgC m<sup>−3</sup> h<sup>−1</sup>). Threshold values of predictors are shown below each split, and variance explained for predictors is shown above each split. Mean values ±1SD and the number of samples (<em>n</em>) are shown for each subset of data, with the three terminal groups identified by numbers in <strong>bold</strong>. The total variation explained by this CART model was 80%. Scatterplot illustrates the relationship between PP and NH<sub>4</sub>–N, with datapoints identified into the three CART groups. The vertical dashed line identifies the NH<sub>4</sub>–N value that best explained variation in PP, and the horizontal line is the PP threshold between the N-limited sites that are limited or not limited by inorganic C.</p> <p><strong>Abstract</strong></p> <p>We compared planktonic primary and secondary production across twenty meltwater ponds on the surface of the McMurdo Ice Shelf in January 2007, including some ponds with basal brines created by meromictic stratification. Primary production ranged from 1.07 to 65.72 mgC m<sup>−3</sup> h<sup>−1</sup> in surface waters. In stratified ponds primary production was always more than ten times higher in basal brines than in the corresponding mixolimnion. Regression tree analysis (<em>r</em><sup>2</sup> = 0.80) identified inorganic nitrogen (as {\mathrm{NH}}_{4}^{+}) as the main factor limiting planktonic primary production. However, there was also evidence of inorganic carbon co-limitation of photosynthesis in some of the more oligotrophic waters. Neither C nor N limited carbon fixation at [NH<sub>4</sub>–N] > 50 mg m<sup>−3</sup>, with photoinhibition the factor most likely limiting photosynthesis under such conditions. Primary production was the only factor significantly correlated to bacterial production and the relationship (<em>r</em><sup>2</sup> = 0.56) was non-linear. Nitrogen limitation and tight coupling of planktonic primary and bacterial production is surprising in these ponds, as all have large pools of dissolved organic carbon (1.2–260 g m<sup>−3</sup>) and organic nitrogen (all >130 mg m<sup>−3</sup>). The dissolved pools of organic carbon and nitrogen appear to be recalcitrant and bacterial production to be constrained by limited release of labile organics from phytoplankton.</p>