Restoration of miR-150 expression enhances Fas-induced apoptosis in miR-150 KO mice.

Eight-week-old miR-150 KO mice were administrated lentiviral particles containing pre-miR-150 (LV-miR-150, 1.01×10⁹ copies/mouse) (n = 6) or lentiviral particles containing scrambled miRNA (LV- scrambled-miRNA, 4.8×10⁸ copies/mouse) (n = 6) by tail vein injection in a volume of 200 μL of sterile saline. 7 days after lentiviral particles injection, the mice were treated with 0.5 μg/g of body weight Jo2 for 4 hours. (A) Hematoxylin and eosin (H&E) staining of the liver tissues (100×, scale bar 20μm). (B) Serum ALT and AST levels. Data are expressed as mean ± SD, *p<0.05. (C) Western blot analysis for the cleavage of PARP and caspases.