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Restoration of PTEN expression attenuates in vitro proliferation of Chordoma cells.
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posted on 2015-08-06, 03:18 authored by Dae-Hee Lee, Ying Zhang, Amin B. Kassam, Myung-Jin Park, Paul GardnerPaul Gardner, Daniel Prevedello, Stephanie Henry, Craig Horbinski, Jan H. Beumer, Hussein Tawbi, Brian J. Williams, Mark E. Shaffrey, Merrill J. Egorin, Roger Abounader, Deric M. Park(A) A representative immunoblot demonstrating reconstitution of PTEN expression via adenoviral delivery. (B) A representative immunoblot for phospho-PDGFRb demonstrating decreased expression with administration of PDGFR inhibitor compared with vehicle controls. (C and D) Cell proliferation assays assessed by the MTS method for C18 cell line (PTEN +/-) and UCH1 cell line (PTEN +/-), respectively, demonstrating decreased proliferation with restoration of PTEN and administration of PDGFR inhibitor. *P<0.05, Two sample independent student’s t test compared with untreated control. **P<0.05, Two sample independent student’s t test compared with PDGFR inhibitor group.
