Representative example of juice reward task (Exp C4, putamen).

(A) Color plot indicating the averaged differential voltammogram of free (left), CS+ (middle) and CS- (right) trials. Abscissa indicates time from juice delivery/CS offset. The ordinate indicates the time from the triangular pulse onset, from the bottom to the top. Note onset of positive response after the juice delivery in free trials (left). Similar response started at -1.5s in the CS+ and CS- trials (middle and right). (B) The current change at the dopamine oxidation peak potential (2.4 ms from the pulse onset). Free (black), CS+ (red with circle) and CS- (blue) trials. Average of 19 to 36 trials are shown with vertical bars indicating s.e.m. Inset on the right shows voltammogram 0.5 s after the free juice onset from averaged differential current (A left). (C) Dopamine-like component extracted by PCR analysis of the same data. (D) In-vivo template waveforms of dopamine and pH extracted from the MFB stimulation experiments as shown in Fig 2. (E) Same as C, but in-vitro template was used instead of in-vivo template. (F) Dopamine and pH waveforms obtained in-vitro, used for the analysis in E. Note the result value (ordinate) from in vivo template (D) is originally given as peak current (C, nA), while concentration (nM) value is directly given (E) from in vitro template (F). Vertical lines in B, C and E indicate onset of timing cue (-2.5s) and onset of CS (-1.5s)