Figure_1.tif (269.63 kB)

Representation of Sge1 homologs in fungi.

figure

posted on 2009-10-23, 02:36 authored by Caroline B. Michielse, Ringo van Wijk, Linda Reijnen, Erik M. M. Manders, Sonja Boas, Chantal Olivain, Claude Alabouvette, Martijn Rep

A) Phylogenetic (neighbour joining, mid-point rooted) tree of Sge1 and FoPac2 with homologs from C. albicans (Wor1 and CAWG_04607), S. pombe (SpGti1 and SpPac2), H. capsulatum (HcRyp1 and HCAG_05432), Magnaporthe grisea (MGG_08850 and MGG_06564), Aspergillus fumigatus (Afu6g04490 and Afu3g09640), Fusarium graminearum (FGSG_12164 and FGSG_10796), Botrytis cinerea (BC1G_11680 and BC1G_14615), Sclerotinia sclerotiorum (SS1G_00031 and SS1G_11157), Cryptococcus neoformans (CNAG_01983 and CNAG_05835), Ustilago maydis (UM05853 and UM06496) and Neurospora crassa (NCU06864), constructed using MacVector software. Only fully aligned parts of the multiple sequence alignment were used (manual curation). Bootstrap percentages are provided only for branches receiving 60% or more support (1000 replications). Branch length reflects the extent of sequence divergence. B) Protein sequence alignment of the Sge1 N-terminal region with H. capsulatum Ryp1, C. albicans Wor1 and S. pombe Gti1. Conserved residues are shaded black, similar residues are shaded grey. The arrow head indicates the conserved threonine residue within the potential protein kinase A phosphorylation site and the asterisk indicates the mutated residue in Sge1R66S. Predicted nucleur localization signals are boxed. The protein sequence alignment were created using VectorNTI software.